A pot experiment was conducted under greenhouse conditions (Nematodes Res. Dept., Plant Pathology Institute, Agricultural Research Center, Giza, Egypt) to evaluate potentialities of Pseudomonas fluorescens, Azotobacter chroococcum, Candida sp. and AM fungi individually or in combination for controlling root-knot nematodes Meloidogyne javanica infected tomato seedlings. The obtained results showed that combined mixture of the tested microorganisms resulted in a pronounced reduction of galls, egg mass and larvae numbers comparing with control treatment. The combined mixture of the tested microorganisms stimulated shoot dry weight, nitrogenase, dehydrogenase and chitinase activities. It was also recorded the highest mycorrhizal infection percentage. Total microbial and yeast counts, as well as macroelements content (NPK %) were determined. These results support the effective role of microorganisms for controlling nematodes blight that infect many vegetable crops.