This work was carried out during 2018/2019 and 2019/2020 seasons at Kom-Ombo Agricultural Research Station (latitude of 24o 28′ N and longitude 32o 57′ E at an elevation108 m above sea level), Aswan Governorate. to assess juice quality and sugar yield of some sugarcane genotypes as affected by bio, inorganic fertilization rates of potassium and nitrogen. The study included 48 treatments, combinations of four genotypes (G.2006-6, G.2003-49, C.57-14 and G.T.54-9), three K rates (48 kg inorganic K2O per fed, 40 kg inorganic K2O + 400-gram bio K and 34 kg inorganic K2O + 800 gram bio K /fed) and four N rates (210 kg inorganic N, 190 kg inorganic N + 300 gram bio N, 170 kg inorganic N + 600 gram bio N and 150 kg inorganic N + 900 gram bio N/fed). A split-split plot design with three replications was used. Results showed that 4 genotypes significantly differed in all studied traits except sugar yield in 1st season. Furthermore, K fertilization had a significant influence on some studied traits. Nitrogen levels had a significant effect on all the studied traits in the two seasons except purity%. Generally, supplying sugar cane G006-6 and G.2003-49 genotypes, with 100% of the recommended inorganic potassium rate (48 kg K2O /fed) integrated with 100% of the recommended inorganic N-rate (210 kg/fed), or 70% of the recommended inorganic potassium rate (34 kg K2O + 800 gram bio K /fed) integrated with 70% of the recommended inorganic N-rate (150 kg inorganic N + 900 gram bio N fed), resulted in the highest sugar yield/fed in plant cane and its first ratoon crops, respectively.