Date palm plants should be slowly acclimatized to ex vitro conditions. Soil application date palm Phoexix dactylifera cv. Malakabi plants by fulvic acid at three concentrations (20, 40 and 80 ml/1) or combined with Arbascular mycorrhizal fungi (AMF) at 3g/pot and N2- fixers mixed cultures (Azospirillum brasilense at 20ml/l and Bacillus polymyxa at 20ml/l) were studied. Addition of AMF once in the beginning of the experiment and addition of fulvic acid and N2-tixers were once every 10 days in the first 6 months and once every 5 days in the second 6 months to enhance plant growth during acclimatization stage. Plants received NPK (2g/l) were used as control treatment. The results cleared that enhancing fulvic acid with AMF and N2 fixers mixed cultures significantly increased vegetative growth (plant height, stem base diameter and total number of leaves/plant), beside increased the biological activity in the date palm rhizosphere (total counts of bacteria, fungi, actinomycetes, Mycorrhizal colonization percentage, nitrogenase and dehydrogenase activities). The studied treatments succeeded to increase the physiological parameters (chlorophyll a and b, carotene and leaf NPK content). However, decrease of soil NPK content was determined. There was an increment in date palm plants growth by increasing the concentration ot fulvic acid from 20 to 80ml/l. Pronounced treatment was achieved by using fulvic acid at 80ml/l combined with N2- fixers and AMF.